ha tag cut run Search Results


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(A) Arc plot summarizing the evolution of the most prominent chromatin states in senescent CD8 + T cells. The edge width is proportional to the number of 200 bp bins undergoing the indicated chromatin state transition. (B) Genome-wide signal intensity heatmaps of H3K4me1, H3K27ac, ATAC-seq, and <t>H3K27me3</t> at active enhancers (top) and unmarked chromatin (bottom) in SA-β-gal-low and SA-β-gal-high CD8 + T cells. The scale bars indicate the normalized signal. (C) Dot plot of the correspondence analysis linking chromatin state transitions to DEG modules from . Significance of the association was calculated using a chi-square test. (D) Genome browser snapshot of the TBKBP1 and TBX21 loci, showing signal tracks for RNA-seq, ATAC-seq, H3K4me1, H3K27ac, and H3K27me3 in SA-β-gal-low and SA-β-gal-high CD8 + T cells. (A–D) CUT&Tag data were generated from SA-β-gal-low and SA-β-gal-high CD8 + T cells from three younger donors. (E–G) Averaged heatmaps of color-coded modules (E and G) and PCA projection (F) of DEGs identified for the indicated comparisons (top of heatmaps and PCA plot). The scale bar (E and G) indicates the row Z score of the regularized log-transformed counts. (H) Functional overrepresentation analysis map showing significant associations of the MSigDB hallmark gene sets for the indicated DEG modules from (G). (I) Upset plot showing the unique and shared DARs for the indicated CD8 + T cell states. (J and K) As in (E) and (G) for the identified DARs of the indicated comparisons. Data for naive and stimulated CD8 + T cells are from GEO: GSE212347 .
H3k27me3, supplied by EpiCypher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ha+tag+cut+run/H3K4me1+Antibody%2C+SNAP-Certified+for+CUT%26RUN+and+CUT%26Tag/pmc12856879-314-32-33
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(A) Arc plot summarizing the evolution of the most prominent chromatin states in senescent CD8 + T cells. The edge width is proportional to the number of 200 bp bins undergoing the indicated chromatin state transition. (B) Genome-wide signal intensity heatmaps of H3K4me1, H3K27ac, ATAC-seq, and <t>H3K27me3</t> at active enhancers (top) and unmarked chromatin (bottom) in SA-β-gal-low and SA-β-gal-high CD8 + T cells. The scale bars indicate the normalized signal. (C) Dot plot of the correspondence analysis linking chromatin state transitions to DEG modules from . Significance of the association was calculated using a chi-square test. (D) Genome browser snapshot of the TBKBP1 and TBX21 loci, showing signal tracks for RNA-seq, ATAC-seq, H3K4me1, H3K27ac, and H3K27me3 in SA-β-gal-low and SA-β-gal-high CD8 + T cells. (A–D) CUT&Tag data were generated from SA-β-gal-low and SA-β-gal-high CD8 + T cells from three younger donors. (E–G) Averaged heatmaps of color-coded modules (E and G) and PCA projection (F) of DEGs identified for the indicated comparisons (top of heatmaps and PCA plot). The scale bar (E and G) indicates the row Z score of the regularized log-transformed counts. (H) Functional overrepresentation analysis map showing significant associations of the MSigDB hallmark gene sets for the indicated DEG modules from (G). (I) Upset plot showing the unique and shared DARs for the indicated CD8 + T cell states. (J and K) As in (E) and (G) for the identified DARs of the indicated comparisons. Data for naive and stimulated CD8 + T cells are from GEO: GSE212347 .
Anti H3k27ac Rabbit Epicypher, supplied by EpiCypher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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EpiCypher anti flag
(A) Arc plot summarizing the evolution of the most prominent chromatin states in senescent CD8 + T cells. The edge width is proportional to the number of 200 bp bins undergoing the indicated chromatin state transition. (B) Genome-wide signal intensity heatmaps of H3K4me1, H3K27ac, ATAC-seq, and <t>H3K27me3</t> at active enhancers (top) and unmarked chromatin (bottom) in SA-β-gal-low and SA-β-gal-high CD8 + T cells. The scale bars indicate the normalized signal. (C) Dot plot of the correspondence analysis linking chromatin state transitions to DEG modules from . Significance of the association was calculated using a chi-square test. (D) Genome browser snapshot of the TBKBP1 and TBX21 loci, showing signal tracks for RNA-seq, ATAC-seq, H3K4me1, H3K27ac, and H3K27me3 in SA-β-gal-low and SA-β-gal-high CD8 + T cells. (A–D) CUT&Tag data were generated from SA-β-gal-low and SA-β-gal-high CD8 + T cells from three younger donors. (E–G) Averaged heatmaps of color-coded modules (E and G) and PCA projection (F) of DEGs identified for the indicated comparisons (top of heatmaps and PCA plot). The scale bar (E and G) indicates the row Z score of the regularized log-transformed counts. (H) Functional overrepresentation analysis map showing significant associations of the MSigDB hallmark gene sets for the indicated DEG modules from (G). (I) Upset plot showing the unique and shared DARs for the indicated CD8 + T cell states. (J and K) As in (E) and (G) for the identified DARs of the indicated comparisons. Data for naive and stimulated CD8 + T cells are from GEO: GSE212347 .
Anti Flag, supplied by EpiCypher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
This H3K36me2 antibody is validated for chromatin profiling of histone H3 lysine 36 dimethylation (H3K36me2), an epigenetic mark deposited primarily by the histone methyltransferase NSD2 (WHSC1/MMSET). H3K36me2 is broadly enriched downstream of transcription start sites
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Image Search Results


(A) Arc plot summarizing the evolution of the most prominent chromatin states in senescent CD8 + T cells. The edge width is proportional to the number of 200 bp bins undergoing the indicated chromatin state transition. (B) Genome-wide signal intensity heatmaps of H3K4me1, H3K27ac, ATAC-seq, and H3K27me3 at active enhancers (top) and unmarked chromatin (bottom) in SA-β-gal-low and SA-β-gal-high CD8 + T cells. The scale bars indicate the normalized signal. (C) Dot plot of the correspondence analysis linking chromatin state transitions to DEG modules from . Significance of the association was calculated using a chi-square test. (D) Genome browser snapshot of the TBKBP1 and TBX21 loci, showing signal tracks for RNA-seq, ATAC-seq, H3K4me1, H3K27ac, and H3K27me3 in SA-β-gal-low and SA-β-gal-high CD8 + T cells. (A–D) CUT&Tag data were generated from SA-β-gal-low and SA-β-gal-high CD8 + T cells from three younger donors. (E–G) Averaged heatmaps of color-coded modules (E and G) and PCA projection (F) of DEGs identified for the indicated comparisons (top of heatmaps and PCA plot). The scale bar (E and G) indicates the row Z score of the regularized log-transformed counts. (H) Functional overrepresentation analysis map showing significant associations of the MSigDB hallmark gene sets for the indicated DEG modules from (G). (I) Upset plot showing the unique and shared DARs for the indicated CD8 + T cell states. (J and K) As in (E) and (G) for the identified DARs of the indicated comparisons. Data for naive and stimulated CD8 + T cells are from GEO: GSE212347 .

Journal: Cell reports

Article Title: Age-independent and targetable transcription factor networks regulating CD8 + T cell senescence in aging humans

doi: 10.1016/j.celrep.2025.116795

Figure Lengend Snippet: (A) Arc plot summarizing the evolution of the most prominent chromatin states in senescent CD8 + T cells. The edge width is proportional to the number of 200 bp bins undergoing the indicated chromatin state transition. (B) Genome-wide signal intensity heatmaps of H3K4me1, H3K27ac, ATAC-seq, and H3K27me3 at active enhancers (top) and unmarked chromatin (bottom) in SA-β-gal-low and SA-β-gal-high CD8 + T cells. The scale bars indicate the normalized signal. (C) Dot plot of the correspondence analysis linking chromatin state transitions to DEG modules from . Significance of the association was calculated using a chi-square test. (D) Genome browser snapshot of the TBKBP1 and TBX21 loci, showing signal tracks for RNA-seq, ATAC-seq, H3K4me1, H3K27ac, and H3K27me3 in SA-β-gal-low and SA-β-gal-high CD8 + T cells. (A–D) CUT&Tag data were generated from SA-β-gal-low and SA-β-gal-high CD8 + T cells from three younger donors. (E–G) Averaged heatmaps of color-coded modules (E and G) and PCA projection (F) of DEGs identified for the indicated comparisons (top of heatmaps and PCA plot). The scale bar (E and G) indicates the row Z score of the regularized log-transformed counts. (H) Functional overrepresentation analysis map showing significant associations of the MSigDB hallmark gene sets for the indicated DEG modules from (G). (I) Upset plot showing the unique and shared DARs for the indicated CD8 + T cell states. (J and K) As in (E) and (G) for the identified DARs of the indicated comparisons. Data for naive and stimulated CD8 + T cells are from GEO: GSE212347 .

Article Snippet: We performed CUT&Tag on 75,000–100,000 sorted CD8 + fSA-βGal low and high from young donors ( n = 3) with the CUTANA CUT&Tag kit (Epicypher) using the following antibodies: H3K4me1 (Epicypher: 13–0057), H3K27me3 (Epicypher: 13–005), H3K27ac (Active Motif: 39,133) and rabbit IgG as a negative control (Epicypher: 13–0042), according to the manufacturer’s instructions.

Techniques: Genome Wide, RNA Sequencing, Generated, Transformation Assay, Functional Assay